Electrospinning/Electrospray

Electric field-induced direct delivery of proteins by a nanofountain probe

작성자
nanonc
작성일
2015-10-06 06:35
조회
4189
We report nanofabrication of protein dot and line patterns using
a nanofountain atomic force microscopy probe (NFP). Biomolecules
are continuously fed in solution through an integrated microfluidic
system, and deposited directly onto a substrate. Deposition is
controlled by application of an electric potential of appropriate
sign and magnitude between the probe reservoir and substrate.
Submicron dot and line molecular patterns were generated with
resolution that depended on the magnitude of the applied voltage,
dwell time, and writing speed. By using an energetic argument and
a Kelvin condensation model, the quasi-equilibrium liquid–air interface
at the probe tip was determined. The analysis revealed the
origin of the need for electric fields in achieving protein transport
to the substrate and confirmed experimental observations suggesting
that pattern resolution is controlled by tip sharpness and
not overall probe aperture. As such, the NFP combines the highresolution
of dip-pen nanolithography with the efficient continuous
liquid feeding of micropipettes while allowing scalability to 1-
and 2D probe arrays for high throughput.
arrays atomic force microscopy nanolithography patterning
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